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The information on housing, people, culture, employment and education that is displayed about Parc Erissey Industrial Estate, New Portreath Road, Redruth, TR16 4HZ is based on the last census performed in the UK in 2021. a) Immunoblots of ectopic human (hu) DDR1 and endogenous mouse DDR1 in cell lysates and medium of E0771-derived cells. ( b) Tumour growth curve of E0771-derived Ddr1-WT, KO+EV, KO+huDDR1 cells (n = 7 tumours/group).( c) Transwell migration assay for purified CD8 + T cells in the presence of conditioned medium from E0771 cells containing endogenous WT DDR1, Ddr1-KO, or Ddr1-KO and ectopic expression of huDDR1 (n = 3 technical repeats). Value of migrated CD8 + T cell number with parental E0771-conditioned medium is set at “1”. ( d) Quantification of CD8 + T cell migration in the presence of DDR1-neutralizing antibodies, using conditioned medium from E0771 Ddr1-KO or KO+huDDR1 cells (IgG: n = 4, #3,#9,#14,#33: n = 2, technical repeats). Control: isotype IgG; anti-DDR1 antibody: #3, #9, #14, and #33. Value of migrated CD8 + T cell number in the far-left column is set at “1”. ( e) Tumour curves treated with control IgG, #3, #9, #14, and #33 (n = 8 tumours/group). Antibody administration started when tumour volume reached approximately 100 mm 3. All p values were compared to the control IgG group and p value as indicated. ( f) Tumours host survival curves of E0771 Ddr1-KO tumour cells with ectopically expressed human (hu) DDR1 in C57BL/6 hosts treated intratumorally with isotype IgG (Ctrl, n = 17, tumours) or anti-DDR1 antibody #9 (n = 18, tumours). ( g) Host body weight treated with control IgG, #3, #9, #14, and #33 (Ctrl n = 4 mice, #3, #9, #14, and #33 n = 4 mice/group). Antibody administration started when tumour volume reached approximately 100 mm 3. Data are presented as mean values +/− SEM. ( h, i) E0771 KO+huDDR1 tumours in C57BL/6 (n = 8 tumours/group, h) and Rag1 −/− hosts (n = 6 tumours/group, i) treated with either isotype IgG or anti-DDR1 #33 antibody. ( j, k) Tumour volume (j) and survival curve (k) of M-Wnt KO+huDDR1 tumours in C57BL/6 mice treated with isotype IgG and anti-DDR1 antibody #9 (n = 10, tumours/group). ( l, m) Tumour growth (l) and survival percentage (m) of AT-3 KO+huDDR1 tumours in C57BL/6 mice treated with isotype IgG and anti-DDR1 antibody #3 (n = 10, tumours/group). Values represent mean ± SEM. p value as indicated. Tumour volumes were examined by two-way ANOVA; survival analysis was examined by log-rank (Mantel–Cox) test, and migration assay were examined by two-tailed Student’s t-test. Kaur, A. et al. Remodeling of the collagen matrix in aging skin promotes melanoma metastasis and affects immune cell motility. Cancer Discov. 9, 64–81 (2019). Gruosso, T. et al. Spatially distinct tumor immune microenvironments stratify triple-negative breast cancers. J. Clin. Invest. 129, 1785–1800 (2019). Vogel, W. F., Aszodi, A., Alves, F. & Pawson, T. Discoidin domain receptor 1 tyrosine kinase has an essential role in mammary gland development. Mol. Cell. Biol. 21, 2906–2917 (2001).van de Sluis B, Rothuizen J, Pearson PL, van Oost BA, Wijmenga C . Identification of a new copper metabolism gene by positional cloning in a purebred dog population. Hum Mol Genet 2002; 11: 165–173. Bejarano, L., Jordao, M. J. C. & Joyce, J. A. Therapeutic targeting of the tumor microenvironment. Cancer Discov. 11, 933–959 (2021). Clark TG, Merriam RW . Diffusible and bound actin in nuclei of Xenopus laevis oocytes. Cell 1977; 12: 883–891. Lu F, Kishida S, Mu P, Huang P, Cao D, Tsubota S et al. NeuroD1 promotes neuroblastoma cell growth by inducing the expression of ALK. Cancer Sci 2015; 106: 390–396.

Tomko, L. A. et al. Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma. Sci. Rep. 8, 12941 (2018). Huang P, Kishida S, Cao D, Murakami-Tonami Y, Mu P, Nakaguro M et al. The neuronal differentiation factor NeuroD1 downregulates the neuronal repellent factor Slit2 expression and promotes cell motility and tumor formation of neuroblastoma. Cancer Res 2011; 71: 2938–2948. Geng H, Wittwer T, Dittrich-Breiholz O, Kracht M, Schmitz ML . Phosphorylation of NF-kappaB p65 at Ser468 controls its COMMD1-dependent ubiquitination and target gene-specific proteasomal elimination. EMBO Rep 2009; 10: 381–386.

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ISA (UK) 701 (Revised November 2019) (Updated January 2020) applies to audits of listed entities, non-listed public interest entities, entities that are required, and those that choose voluntarily to report on how they have applied the UK Corporate Governance Code, where the auditor is required by law or regulation to communicate key audit matters in the auditor’s report, as well as where the auditor voluntarily chooses to do so. Audit report implications Xia PY, Wang S, Huang GL, Zhu PP, Li M, Ye BQ et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 2014; 211: 2119–2134. Molenaar JJ, Koster J, Zwijnenburg DA, van Sluis P, Valentijn LJ, van der Ploeg I et al. Sequencing of neuroblastoma identifies chromothripsis and defects in neuritogenesis genes. Nature 2012; 483: 589–593. Lim KL, Chew KCM, Tan JMM, Wang C, Chung KKK, Zhang Y et al. Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation. J Neurosci 2005; 25: 2002–2009.

Lu F, Zheng Y, Donkor PO, Zou P, Mu P . Downregulation of CREB promotes cell proliferation by mediating G1/S phase transition in Hodgkin Lymphoma. Oncol Res 2016; 24: 171–179.Harbour ME, Breusegem SY, Seaman MNJ . Recruitment of the endosomal WASH complex is mediated by the extended 'tail' of Fam21 binding to the retromer protein Vps35. Biochem J 2012; 442: 209–220. Salmon, H. et al. Matrix architecture defines the preferential localization and migration of T cells into the stroma of human lung tumors. J. Cell. Invest. 122, 899–910 (2021).

Department of Anatomy and Cell Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC, USA Zhao XY, Liang SF, Yao SH, Ma FX, Hu ZG, Yan F et al. Identification and preliminary function study of Xenopus laevis DRR1 gene. Biochem Biophys Res Commun 2007; 361: 74–78.Huang Y, Wu M, Li HY . Tumor suppressor ARF promotes non-classic proteasome-independent polyubiquitination of COMMD1. J Biol Chem 2008; 283: 11453–11460.